Zfy2^em2Asah

Exon 4 was targed for modification using the CRISPR/Cas9 system. The sgRNA target sequence was 5'-GAAGCAGTCTTAGATTCCAGtgg-3' (PAM sequence in lower case). As this gRNA targets identical sequence in this gene and in Zfy1, the procedure yielded single knock-outs (one founder for each gene) as well as double knock-outs (10 founders). Genotyping of this knock-out allele found in double knock-out founder #1 revealed a 1 bp deletion in the target sequence. (J:238696)