这个靶向载体包含了一个由loxP切割位点介导的PGKneo cassette,位于Biotin-3xFLAG(编码生物素识别序列和3个FLAG抗原决定簇)序列下游,该序列连接在基因的C端。通过Cre酶介导的重组,去除了含floxed neo cassette的元件。 Western blot结果显示Rnx2-BioFL蛋白的水平与野生型相似。(来源:J:328555)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
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1
4
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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