Pdx1^tm1.1Cvw

Using the Pdx1tm1Mgn Loxed Cassette Acceptor allele as a starting point, the cassette was replaced with another cassette via cre recombination mediated cassette exchange. The replacement cassette is comprised of: forward lox71 site, an FRT site flanked hygromycin resistance gene cassette, the genomic sequence encompassing the cis-regulatory area IV, I and III regions and exon 1, and a reverse lox2272 site. After removal of the hygro cassette through flp-mediated recombination this leaves an allele with a deletion of 256 bp upstream of exon 1 wich includes the entire area II. (J:238418)