Ctbp2^tm1.2Sud

The endogenous N-terminal Ribeye A-domain was replaced with a loxP site followed by the Ribeye A domain cDNA fused to GCaMP3 (a GFP based calcium sensor). The coding region is followed by a polyA signal, an FRT-flanked PGK-neomycin selection cassette, and a 3' prime loxP site. The neo cassette is subsequently removed through flp-mediated recombination. The floxed tagged A-domain was deleted through cre-mediated recombination. This removes the possibility of transcribing the Ribeye isoform but leaves the other isoforms intact. (J:233453)