设计了一种靶向载体,其包含一个在第3外显子上游的-loxP位点,接着是FRT引导的诺卡因抗性(neo)基因座,随后是一个位于第4外显子下游的-loxP位点。通过Flp介导的重组,FRT引导的neo基因座被移除,使得3号和4号外显子成为可剪接的区域。随后的Cre介导重组在生殖细胞中导致了3号和4号外显子的消除。(来源:J:229955)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入,基因内删除
--
1
--
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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