Tg(TAGLN-NOTCH3*R90C)1Etl

A 7021-bp full-length human Notch3 cDNA carrying the codon 90 CGT to TGT mutation was ligated to a 2191-bp fragment of the TAGLN gene promoter, including 2126 bp upstream of the initiation transcription site and the complete noncoding first exon. A 584 bp fragment of the bovine beta-globin intron was inserted between the SM22alpha regulatory sequence and the Notch3 sequence. A polyadenylation signal was derived from the SV40 poly(A) DNA and was inserted at the 3 end of the construct. Three lines were generated; lines Ma (1, mauve) and Ve (2, verte) were used for further analysis. (J:129831)