Ano6^tm1.2Phep

A loxP site was inserted located 5 of exon 13, and a neomycin resistance (neor) gene flanked with two frt sites, and a loxP site were inserted 3 of exon 13. Mice were crossed with FLP-expressing and Cre-expressing transgenic mice to remove the frt flanked neoR cassette and loxP flanked exon 13, resulting in a frame-shift mutation in exon 14. (J:238366)