Sf3b1^tm1.1Mdf

The targeting vector contains (from 5' to 3') a mutant loxP site (upstream of exon 15), and downstream of exon 16 - a FRT-flanked neomycin/lacZ cassette, a loxP site, inverted copies of exon 15 with the K700E mutation and exon 16, a second mutant loxP site (in opposite orientation to the first mutant loxP site) and a loxP site (in opposite orientation to the first loxP site). Flp-mediated recombination removed the FRT-flanked neo cassette. The K700E (lysine to glutamic acid) substitution is associated with myelodysplastic syndromes. The unrecombined conditional allele is hypomorphic. (J:234976)