一个LoxP-4XPolyA-LoxP-H2B-tdTomato-FRT-Neo-FRT的 cassette被定向到kit基因座的起始密码子位置,通过同源重组在ES细胞中去除了内源性ATG。通过Flp介导的重组,Neo选择性 cassette被移除,留下一个单独的FRT位点。随后,这个突变小鼠品系与Protamine-Cre小鼠杂交,以去除4xPloyA的终止子 cassette。(来源:J:227907)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129
Targeted
插入
--
1
27
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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