针对基因的Exons 3和4被替换为一个构建,内容包括:loxP-FRT-polyA-neomycin(反向转录方向)-FRT-Exons 3-9的Pofut2迷你cDNA-polyA-loxP-Stop-mCherry-polyA。通过Flp介导的重组,FRT标记的neo cassette被去除了。这个等位基因不产生mCherry荧光。通过Cre介导的重组,loxP标记的Exons 3-9也被移除。体内原位杂交和HE染色结果显示,胚胎无法表达胎外中胚层和内胚层分化相关的标记。(来源:J:234992)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
B6.Cg-Thy1a
Targeted
插入,基因内删除
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1
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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