Camk2a^em1Rjco

Using the CRISPR/Cas9 system, the Glu183 codon GAA was targeted and changed to valine codon GTG with the aid of a donor DNA sequence and sgRNA CTCCCCAGAAGTGCTGAGGAAAG. This mutation, in the catalytic domain of the gene, models a novel mutation in a human autism spectrum disorder (ASD) patient. Immunoblots showed a marked reduction in peptide levels for this gene in the forebrain of hetero- and homozygous mutants. (J:239596)