A targeting vector containing an FRT-flanked PuroR selection cassette was used to insert S910A and S935A point mutations into exon 21. Flp-mediated recombination removed the FRT-flanked PuroR cassette. (J:237182)
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A targeting vector containing an FRT-flanked PuroR selection cassette was used to insert S910A and S935A point mutations into exon 21. Flp-mediated recombination removed the FRT-flanked PuroR cassette. (J:237182)