Usp15^em1Nki

CRISPR/Cas9 system was used to generate a 91,795 bp deletion using 5 gRNA cut at Chr10:123,196,931 upstream of exon 1 and a 3 gRNA cut at Chr10:123,105,136, downstream of the last exon, exon 22. The sequence at the fusion point is: gccgccgcctccgctaTcGGatcgtatcaagtgacat (capital letters: different from expected fusion product). (J:306500)