The targeting construct is designed from 5' to 3' as follows: CAG-loxP-neomycin (in reverse transcriptional orientation)-Stop-loxP-human BST2/tetherin cDNA-FRT-IRES-EGFP-FRT-polyA and was introduced to intron 1 of the mouse Gt(ROSA)26Sor locus. (J:101977)