CRISPR/Cas技术针对exon 2进行靶向,产生了缺失23个核苷酸的敲除等位基因。 Western blot分析证实了睾丸中蛋白质水平减少了80%。(来源:J:233657)
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CRISPR/Cas technology targeted exon 2 to generate a knock-out allele lacking 23 nucleotides. Western blot analysis confirmed an 80% reduction in protein levels in the testes. (J:233657)