L1L2_gt1 cassette被插入到染色体2的160,511,599位点,位于关键外显子(Build GRCm39)上游。这个 cassette 包含FRT引导的lacZ/诺卡因序列,后面跟着一个loxP位点。在目标外显子(s)下游,再插入了一个loxP位点,位置是160,512,356。通过小鼠中flp重组酶的表达,创建了一个“条件准备”(含flipped)等位基因,去除了lacZ序列和诺卡因选择性载体。关键外显子(s)仍被loxP位点包围。(来源:J:236045)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6N
Targeted
插入
--
1
--
3

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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