Top1^{tm1c(KOMP)Wtsi}

The L1L2_gt1 cassette was inserted at position 160511599 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 160512356. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ sequences and neomycin selection cassette. The critical exon(s) is/are remain flanked by loxP sites. (J:236045)