Tg(PDGFB-LRRK2*R1441C)31Hlw

Human LRRK2 with the disease causing R1441C mutation with an HA tag on the C-terminus is under the control of the CMV enhancer/platelet-derived growth factor-beta chain promoter. Immunoblot analysis confirmed transgene expression in various brain regions, including the cerebral cortex, substantia nigra, and striatum. Western blot analysis indicates a 2-fold overexpression of the protein compared to the endogenous level in the substantia nigra. Two lines, 31 and 39, were established and line 31 is entered as the representative line. (J:229559)