Shank3^tm1.2Yhj

A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site were inserted into intron 9. Subsquently, an FRT site flanked puromycin resistance gene cassette and a third loxP site were inserted downstream of exon 22. Subsequent flp-mediated recombination removed the two selection cassettes and cre-mediated recombination deleted exons 4 to 22, creating a null allele. (J:235641)