The targeting construct contains a tetO (tetracycline response element; TRE)-loxP-Stop-loxP-Optopatch3-CAG-tTA (tetracycline-controlled transactivator). Optopatch3 is a bicistronic construct that coexpresses QuasAr3-mCitrine (an enhanced archaerhodopsin-3 (Arch)-based voltage indicator) and CheRiff (a sensitive blue-shifted optogenetic actuator) (J:101977)