Kmo^{tm1c(KOMP)Wtsi}

The L1L2_Bact_P cassette was inserted at position 175470673 of Chromosome 1 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 175471397. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ sequences and the neomycin selection cassette. Exon 4 remains flanked by loxP sites. (J:233151)