在第7和8号外显子之间插入了一个loxP位点,然后在第3和4号外显子间插入了含frt座的诺卡因筛选 cassette,其中也包含一个loxP位点。 Western blot结果显示蛋白表达量降低。(来源:J:226703)
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A single loxP site was introduced between exons 7 and 8 and a neomycin selection cassette flanked by frt sites with a single loxP site was inserted between exons 3 and 4. Western blot analysis shows attenuated protein levels. (J:226703)