设计了一种靶向载体,其在exon 2上游插入了loxP位点,下游包含FRT-splice捐赠/接受者-IRES-β-gal/neo-FRT-loxP序列。将这种载体短暂转染到ES细胞中,通过表达FLP的质粒来去除SA-IRES-β-gal基因元件,从而实现exons 2-3区域的去粘连。(来源:J:234617)
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cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count