设计了一种靶向载体,其在exon 2上游插入了loxP位点,下游包含FRT-splice捐赠/接受者-IRES-β-gal/neo-FRT-loxP序列。将这种载体短暂转染到ES细胞中,通过表达FLP的质粒来去除SA-IRES-β-gal基因元件,从而实现exons 2-3区域的去粘连。(来源:J:234617)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae
Targeted
插入
--
1
--
4

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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