Log In|Sign Up
中文
设计了一个靶向载体，包含 Cre/ERT2 粘合基因、SV40 polyA 启动子信号和Frt标记的neo-polyA插件，目标插入Gdf5基因的起始翻译位点。这个载体通过电穿孔技术导入了C57BL/6来源的胚胎干细胞（ES细胞）。经过定向筛选的ES细胞被注入受体囊胚，然后与携带白化C57BL/6J转基因ROSA26::FLPe敲入雌性的杂交小鼠交配，以剔除neo选择性元件并建立克隆。(引用：J:234264)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
