一个PGK-氨霉素表达载体已经替换了基因的1.5kb5'非编码区和前3个外显子。RT-PCR检测显示这些区域没有基因转录, Western blot分析证实了在纯合突变小鼠肺组织中没有蛋白质表达。对2倍稀释的鼠血清进行的定量ELISA也未检测到蛋白质,免疫组织化学分析进一步证实了动脉组织中未发现该蛋白质。(引用文献:J:233818)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S1/Sv-Oca2+ Tyr+ Kitl+
Targeted
插入,基因内删除
--
1
--
5

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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