FRT-flanked的β-geo cassette替换了exon 2,其中包含furin切割位点。通过Flp介导的重组移除了这个β-geo cassette。 Western blot结果显示E18.5大脑中没有蛋白表达(来源:J:206855)
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An FRT-flanked beta-geo cassette replaced exon 2, which contains the furin cleavage site. Flp-mediated recombination removed the beta-geo cassette. Western blot analysis confirmed the absence of protein expression in E18.5 brains. (J:206855)