在开放阅读框上游插入了一个loxP位点。接着插入了一个V5抗原标签,与开放阅读框连接在一起。通过Flp介导的重组,FRT引导的诺卡因抗性基因座被移除,其中在3' UTR中插入了一个3'的loxP位点。随后,Cre介导的重组去除了开放阅读框。(来源:J:228526)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S6/SvEvTac x C57BL/6NCrl)F1
Targeted
插入,基因内删除
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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