在lacZ和floxedneo抗药性基因座取代了大部分exon 1后,通过Cre介导的重组移除了选择性元件。 Western blot结果显示新生仔猪后肢肌肉中没有蛋白质表达(来源:J:227534)
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A lacZ and floxed neomycin resistance cassette replaced most of exon 1. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of protein expression in neonatal hind limb muscle. (J:227534)