一个敲除的诺卡因抗性基因座替换了编码序列。通过Cre介导的重组,选择性座舱被移除。 Western blot分析证实了大脑中没有蛋白质存在。(来源:J:227160)
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A floxed neomycin resistance cassette replaced the coding sequence. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of protein in the brain. (J:227160)