Col1a1^{tm20(tetO-GFP,-cas9*D10A)Slowe}

RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a paired guide RNAs targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence followed by a tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease nickase variant cas9D10A (Cas9n; derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence. (J:222595)