Col1a1^{tm17(tetO-GFP,-cas9*)Slowe}

RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a single guide RNA targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence and an tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease cas9 (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence. (J:222595)