Ino80^tm1.2Jland

The targeting vector is designed to have a loxP site upstream of exon 2, and an FRT-flanked neo cassette followed by a second loxP site just downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neo cassette. Germ line, cre mediated recombination removed exons 2 through 4. Western blot analysis confirmed that no protein was expressed in mouse embryo fibroblasts. Two small transcripts are observed using RT-PCR. Sequencing identified these as aberrant exon 1-5 and exon 1-6 splice events. The first ATG of each splice product results in an out of frame transcript and a random protein product. (J:229516)