Lmna^tm11Lgf

Introns 10 and 11 and the last 150 nucleotides of exon 11 were deleted, thereby abolishing production of lamin C and producing a mutated prelamin A that precludes wild-type Lmna synthesis. The lamin C 3' UTR replaced the endogenous prelamin A 3' UTR and a floxed neomycin resistance cassette was inserted downstream of the modified 3' UTR. Absence of a Mir9 binding site results in expression in neurons. High levels of expression are detected in the CNS and peripheral tissues. (J:220988)