ENU诱变导致了 intron 3中的G变A,产生了一个27个核苷酸的内含子插入,编码了9个非活性氨基酸。 Western blot分析证实了E10.5胚胎中蛋白质表达水平的降低。(来源:J:223082)
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ENU mutagenesis induced a G to A transition in intron 3 that results in an in-frame 27 nucleotide insertion encoding 9 additional neutral amino acids. Western blot anlaysis confirmed reduced protein expression in E10.5 embryos. (J:223082)