Exon 11被替换为一个带有FRT和loxP切割位点的诺卡因抗性载体,同时对修改后的Exon 11进行了一点突变,将苏氨酸(S)替换为阿尔法半胱氨酸(S557A)。通过Flp介导的重组,去除了选择性载体。在纯合突变小鼠肝脏中表达的CRY2蛋白,对识别S557磷酸化的CRY2抗体没有免疫反应。(来源:J:224319)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6NCrlj x CBA/JNCrlj)F1
Targeted
Insertion, Nucleotide substitutions
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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