A loxP site and FRT flanked neo cassette were inserted upstream of exon 1 and a loxP site was inserted downstream of exon 1 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exon 1. (J:220676)
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A loxP site and FRT flanked neo cassette were inserted upstream of exon 1 and a loxP site was inserted downstream of exon 1 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exon 1. (J:220676)