Tg(CAG-en/Hoxb5,-EGFP)#Vchl

The transgene contains a CAG promoter, floxed chloramphenicol acetyl transferase gene, a fusion gene in which the Drosophila engrailed repressor domain replaces the transcription activation domain of mouse Hoxb5, an IRES sequence and an EGFP reporter gene. Cre-mediated recombination is required to achieve expression of the fusion gene. Two lines (A2 and E4) achieved germline transmission. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:136122)