Zfyve26^tm1.1Cahb

A neomycin cassette (Neo) flanked by frt sites and a loxP-site was inserted into intron 15. A second loxP-site together with a BamHI site was introduced into intron 14. Cre-mediated recombination removed exon 15 and the selection cassette. Northern blot analysis of total brain RNA from control and homozygous mutant animals showed that no expressed transcript is detectable. Western blot analysis with affinity purified antibodies against the N- terminus or the C-terminus of the encoded protein detected a polypeptide in brain extracts from wild-type but not from homozygous mutant mice. (J:223127)