Samd8^tm2Kwi

The gene has been modified by insertion of a loxP site upstream of exon 5, a frt-flanked SV40-neo cassette followed by a second loxP site downstream of the 3' UTR, and a second copy of exon 5 followed in-frame by the EGFP coding sequence. Cre recombinase-mediated excision of the endogenous exon 5 through the 3' untranslated region will result in splicing of exon 4 to the downstream copy of exon 5, leading to production of a SAMD8/EGFP fusion protein lacking the catalytic domain encoded by exon 6. (J:228568)