在exon 3'的上游插入了loxP位点,接着是含氨苄青霉素抗性基因座的FRT序列,再在exon 3'的下游也插入了loxP位点。随后通过flp介导的重组,去除了neo cassette,得到了一个条件性可操作的等位基因,其exon 3'编码的deltaN亚型被-loxP标记。(来源:J:218385)
Basic Information
(129S6/SvEvTac x C57BL/6NCrl)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count