TALEN介导的重组在exon 1中插入了tdTomato报告基因和三重polyadenylation序列。tdTomato荧光在骨骼肌中特异性检测到,而在如肌腱等其他组织中未见。实时PCR,使用针对下游exons 2和3的特定引物,证实了骨骼肌和肝脏中都没有转录本。(来源:J:217579)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x C3H)F1
Endonuclease-mediated
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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