Tg(hs1056-cre/ERT2,-GFP)1705Jlr

This transgene contains the human enhancer element hs1056, which is flanked on human Chr 18 by the long intergenic non-protein coding RNA 1029 (LINC01029) and spalt-like transcription factor 3 (SALL3) loci, followed by a minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus. (J:94077)