Tg(hs953-cre/ERT2,-GFP)526Jlr

This transgene contains the human enhancer element hs953, which is flanked on human Chr 2 by the Sp9 transcription factor (SP9) and corepressor interacting with RBPJ, 1 (CIR1) genes, followed by a minimal Hsp68 promoter, the cre/ERT2 fusion gene, an internal ribosomal entry site (IRES), and a green fluorescent protein reporter gene. The fusion gene encodes Cre recombinase joined to a modified ligand-binding domain of the human estrogen receptor that preferentially binds tamoxifen or 4-hydroxytamoxifen, but not endogenous 17beta-estradiol; upon binding to tamoxifen, the cre/ERT2 protein is translocated to the nucleus. (J:94077)