Tg(tetO-EGFR*T790M*L858R)51Paow

A mutant human epidermal factor receptor cDNA with a C-to-T nucleotide substitution in exon 20 that replaces threonine with methionine at amino acid position 790 (T790M) and a T-to-G nucleotide substitution in exon 21 that replaces leucine with arginine at amino acid position 858 (L858R) was cloned between a tetracycline-responsive element and an intron and polyadenylation signal from the mouse protamine 1 gene. Mice with this transgene that express the tetracycline transactivator (tTA) or reverse transactivator (rtTA) under control of a tissue-specific promotor can be induced to express the mutant gene in tissues or cell types of interest by administration/withdrawal of doxycycline. Three founders were established: 12, 29, and 51, and line 51 is entered as a representative line. (J:128700)