一个包含整个基因的1373bp区域通过同源重组被PGK-neo cassette替换。RT-PCR和 Western blot分析证实了在纯合突变小鼠中确实存在这一基因的缺失。(来源:J:217318)
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A 1373 bp region including the entire gene was replaced with a PGK-neo cassette via homologous recombination. RT-PCR and Western blot analysis confirmed the deletion in homozygous mutant mice. (J:217318)