Tg(PGK1-FTL*)#Sle

The constitutive and ubiquitous human PGK1 promoter drives the expression of the human cDNA bearing the 498-499InsTC mutation causing the substitution of the last 9 amino acids and an elongation of extra 16 amino acids at the C-terminus of L-ferritin peptide. This modification reduces the ability of the protein to store iron. ELISA and Immunoblotting confirm high expression of the transgene in the liver and brain. Although three lines were obtained, only one was used for further analysis. However, line numbers were not identified and the pound (#) symbol is used because the line number is not specified. (J:227451)