Gt(ROSA)26Sor^{tm3(CAG-tdTomato,-EGFP*)Zjh}

The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, an FRT-flanked tdTomato sequence (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), three copies of green fluorescent protein, and a Neo-polyA cassette. The three GFP sequences include emerald, TagGFP (Aequorea macrodactyla GFP), and hrGFP (Humanized Renilla reniformis GFP) variants. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:235123)