靶向载体设计用于插入内源性核糖体进入位点(IRES),连接到经过优化的FLPe基因(用于哺乳动物细胞中翻译和细胞及小鼠中更高重组效率的FLpe基因),后面跟着一个由loxP序列标记的neo cassette,位于基因的终止密码子下游。通过Cre介导的重组,去除了含floxed neo cassette的部分。(来源:J:235123)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(C57BL/6 x 129S4/SvJae)F1
Targeted
插入
--
1
8
9

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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