设计了一种靶向载体,其在基因的第4个内含子中插入了loxP位点,紧接着是编辑序列的互补序列(ECS)的loxp座标,然后是loxp引导的诺卡因抗性(neo)基因片段,这个片段被loxp标记。通过Cre介导的重组,去除了含floxed neo标记的片段。(来源:J:144148)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count