Scn1a^tm1.1Swl

A premature stop codon (CAG to TAG) at amino acid residue E1099 (E1099X) was introduced into the linker between domains 2 and 3. The loxP flanked neo cassette downstream of the mutation was removed via cre-mediated recombination. Western blot analysis indicates that global protein expression is reduced to 54.1% in heterozygotes and is undetectable in homozygotes, indicating a null allele. (J:221045)