将包含IRES-cre cDNA序列和frt-flanked PGK-hygromycin筛选标记的磁带插入Nfatc1基因3' UTR(在终止密码子后),在R1 ES细胞中实现了精确靶向。正确着色的细胞被选中并扩增,然后进行囊胚注射。(来源:J:193198)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count